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pce hul 11 2 kbp  (Addgene inc)


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    Addgene inc pce hul 11 2 kbp
    Pce Hul 11 2 Kbp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pce+hul+11+2+kbp/pCE-hSK+(Plasmid+%2341814)/pm41344296-10-120-123
    Average 93 stars, based on 37 article reviews
    pce hul 11 2 kbp - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Biallelic excision of the CTG18.1 expansion in two Fuchs endothelial corneal dystrophy-derived iPSC lines and one control (SCTCi046-A-1, SCTCi047-A-1 and SCTCi041-A-1) using an episomal vector-based CRISPR/Cas9 approach.
    Article Snippet: .. Unique stem cell line identifier SCTCi041-A-1 https://hpscreg.eu/cell-line/SCTCi041-A-1 SCTCi046-A-1 https://hpscreg.eu/search?q=SCTCi046-A-1 SCTCi047-A-1 https://hpscreg.eu/search?q=SCTCi047-A-1 Alternative name(s) of stem cell line IPS22-00053 Δ/Δ CTG18.1 clone 6 IPS22-00052 Δ/Δ CTG18.1 clone 2 IPS23-00122 Δ/Δ CTG18.1 clone 29 Institution Radboud University Medical Centre Contact information of the reported cell line distributor Hans van Bokhoven hans.vanbokhoven@radboudumc.nl Type of cell line iPSC Origin Human Additional origin info (applicable for human ESC or iPSC) SCTCi041-A-1 Age: 78 y/o Sex: Female Ethnicity: Caucasian SCTCi046-A-1 Age: 67 y/o (continued on next column) Resource Table (continued ) Sex: Female Ethnicity: Caucasian SCTCi047-A-1 Age: 72 y/o Sex: Female Ethnicity: Caucasian Cell Source Peripheral blood mononuclear cell (PBMC) Method of reprogramming Episomal (hSOX2, hKLF4, hL-MYC, hLIN28, OCT3/4) Reprogramming plasmids: pCE-hSK 11.9 kbp (Addgene #41814) pCE-hUL 11.2 kbp (Addgene #41855) pCE-hOCT3/4 10.5 kbp (Addgene #41813) Clonality Clonal Evidence of the reprogramming transgene loss (including genomic copy if applicable) Loss of transgene reprogramming was confirmed with genomic DNA PCR of EBNA1 (Supplementary figure 1B) The cell culture system used E8 Flex Type of the Genetic Modification Induced mutation: CTG18.1 biallelic excision (continued on next page) * Corresponding author. ..

    Cell Culture:

    Article Title: Biallelic excision of the CTG18.1 expansion in two Fuchs endothelial corneal dystrophy-derived iPSC lines and one control (SCTCi046-A-1, SCTCi047-A-1 and SCTCi041-A-1) using an episomal vector-based CRISPR/Cas9 approach.
    Article Snippet: .. Unique stem cell line identifier SCTCi041-A-1 https://hpscreg.eu/cell-line/SCTCi041-A-1 SCTCi046-A-1 https://hpscreg.eu/search?q=SCTCi046-A-1 SCTCi047-A-1 https://hpscreg.eu/search?q=SCTCi047-A-1 Alternative name(s) of stem cell line IPS22-00053 Δ/Δ CTG18.1 clone 6 IPS22-00052 Δ/Δ CTG18.1 clone 2 IPS23-00122 Δ/Δ CTG18.1 clone 29 Institution Radboud University Medical Centre Contact information of the reported cell line distributor Hans van Bokhoven hans.vanbokhoven@radboudumc.nl Type of cell line iPSC Origin Human Additional origin info (applicable for human ESC or iPSC) SCTCi041-A-1 Age: 78 y/o Sex: Female Ethnicity: Caucasian SCTCi046-A-1 Age: 67 y/o (continued on next column) Resource Table (continued ) Sex: Female Ethnicity: Caucasian SCTCi047-A-1 Age: 72 y/o Sex: Female Ethnicity: Caucasian Cell Source Peripheral blood mononuclear cell (PBMC) Method of reprogramming Episomal (hSOX2, hKLF4, hL-MYC, hLIN28, OCT3/4) Reprogramming plasmids: pCE-hSK 11.9 kbp (Addgene #41814) pCE-hUL 11.2 kbp (Addgene #41855) pCE-hOCT3/4 10.5 kbp (Addgene #41813) Clonality Clonal Evidence of the reprogramming transgene loss (including genomic copy if applicable) Loss of transgene reprogramming was confirmed with genomic DNA PCR of EBNA1 (Supplementary figure 1B) The cell culture system used E8 Flex Type of the Genetic Modification Induced mutation: CTG18.1 biallelic excision (continued on next page) * Corresponding author. ..

    Modification:

    Article Title: Biallelic excision of the CTG18.1 expansion in two Fuchs endothelial corneal dystrophy-derived iPSC lines and one control (SCTCi046-A-1, SCTCi047-A-1 and SCTCi041-A-1) using an episomal vector-based CRISPR/Cas9 approach.
    Article Snippet: .. Unique stem cell line identifier SCTCi041-A-1 https://hpscreg.eu/cell-line/SCTCi041-A-1 SCTCi046-A-1 https://hpscreg.eu/search?q=SCTCi046-A-1 SCTCi047-A-1 https://hpscreg.eu/search?q=SCTCi047-A-1 Alternative name(s) of stem cell line IPS22-00053 Δ/Δ CTG18.1 clone 6 IPS22-00052 Δ/Δ CTG18.1 clone 2 IPS23-00122 Δ/Δ CTG18.1 clone 29 Institution Radboud University Medical Centre Contact information of the reported cell line distributor Hans van Bokhoven hans.vanbokhoven@radboudumc.nl Type of cell line iPSC Origin Human Additional origin info (applicable for human ESC or iPSC) SCTCi041-A-1 Age: 78 y/o Sex: Female Ethnicity: Caucasian SCTCi046-A-1 Age: 67 y/o (continued on next column) Resource Table (continued ) Sex: Female Ethnicity: Caucasian SCTCi047-A-1 Age: 72 y/o Sex: Female Ethnicity: Caucasian Cell Source Peripheral blood mononuclear cell (PBMC) Method of reprogramming Episomal (hSOX2, hKLF4, hL-MYC, hLIN28, OCT3/4) Reprogramming plasmids: pCE-hSK 11.9 kbp (Addgene #41814) pCE-hUL 11.2 kbp (Addgene #41855) pCE-hOCT3/4 10.5 kbp (Addgene #41813) Clonality Clonal Evidence of the reprogramming transgene loss (including genomic copy if applicable) Loss of transgene reprogramming was confirmed with genomic DNA PCR of EBNA1 (Supplementary figure 1B) The cell culture system used E8 Flex Type of the Genetic Modification Induced mutation: CTG18.1 biallelic excision (continued on next page) * Corresponding author. ..

    Article Title: Biallelic excision of the CTG18.1 expansion in two Fuchs endothelial corneal dystrophy-derived iPSC lines and one control (SCTCi046-A-1, SCTCi047-A-1 and SCTCi041-A-1) using an episomal vector-based CRISPR/Cas9 approach.
    Article Snippet: .. Resource Table (continued ) Associated disease CORNEAL DYSTROPHY, FUCHS ENDOTHELIAL, 3; FECD3 OMIM #613267 Gene/locus modified in the reported transgenic line TCF4-CTG18.1 Cytogenetic location: 18q21.2 Genomic coordinates (GRCh38): (GRCh38): 18:55222185-55635957 Method of modification / usercustomisable nucleases (UCN) used, the resource used for design optimisation UCN: CRISPR/Cas, SpCas9 Software used for choosing gRNA: CRISPOR User-customisable nuclease (UCN) delivery method Encoding plasmid transfection All double-stranded DNA genetic material molecules introduced into the cells Reprogramming plasmids: pCE-hSK 11.9 kbp (Addgene #41814) pCE-hUL 11.2 kbp (Addgene #41855) pCE-hOCT3/4 10.5 kbp (Addgene #41813) CRISPR/Cas9 gRNA plasmids: pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene #62988) pSpCas9(BB)-2A-GFP (PX458) (Addgene #48138) Evidence of the absence of random integration of any plasmids or DS DNA introduced into the cells. .. Reprogramming plasmids: EBNA1-PCR CRISPR/Cas9 gRNA plasmids: CRISPR/Cas9-PCR Analysis of the nuclease-targeted allele status Sanger sequencing of the targeted alleles Homozygous allele status validation N/A Method of the off-target nuclease activity prediction and surveillance Targeted the top 4 off-target genomic site predicted by CRISPOR for both gRNA-01 and gRNA-02 Descriptive name of the transgene N/A Eukaryotic selective agent resistance cassettes (including inducible, gene/cell type-specific) N/A Inducible/constitutive expression system details N/A Date archived/stock creation date 26th August 2023 Cell line repository/bank N/A Ethical/GMO work approvals This study was GMO approved by Radboudumc (IG05-145) Addgene/public access repository recombinant DNA sources’ disclaimers (if applicable) N/A

    Mutagenesis:

    Article Title: Biallelic excision of the CTG18.1 expansion in two Fuchs endothelial corneal dystrophy-derived iPSC lines and one control (SCTCi046-A-1, SCTCi047-A-1 and SCTCi041-A-1) using an episomal vector-based CRISPR/Cas9 approach.
    Article Snippet: .. Unique stem cell line identifier SCTCi041-A-1 https://hpscreg.eu/cell-line/SCTCi041-A-1 SCTCi046-A-1 https://hpscreg.eu/search?q=SCTCi046-A-1 SCTCi047-A-1 https://hpscreg.eu/search?q=SCTCi047-A-1 Alternative name(s) of stem cell line IPS22-00053 Δ/Δ CTG18.1 clone 6 IPS22-00052 Δ/Δ CTG18.1 clone 2 IPS23-00122 Δ/Δ CTG18.1 clone 29 Institution Radboud University Medical Centre Contact information of the reported cell line distributor Hans van Bokhoven hans.vanbokhoven@radboudumc.nl Type of cell line iPSC Origin Human Additional origin info (applicable for human ESC or iPSC) SCTCi041-A-1 Age: 78 y/o Sex: Female Ethnicity: Caucasian SCTCi046-A-1 Age: 67 y/o (continued on next column) Resource Table (continued ) Sex: Female Ethnicity: Caucasian SCTCi047-A-1 Age: 72 y/o Sex: Female Ethnicity: Caucasian Cell Source Peripheral blood mononuclear cell (PBMC) Method of reprogramming Episomal (hSOX2, hKLF4, hL-MYC, hLIN28, OCT3/4) Reprogramming plasmids: pCE-hSK 11.9 kbp (Addgene #41814) pCE-hUL 11.2 kbp (Addgene #41855) pCE-hOCT3/4 10.5 kbp (Addgene #41813) Clonality Clonal Evidence of the reprogramming transgene loss (including genomic copy if applicable) Loss of transgene reprogramming was confirmed with genomic DNA PCR of EBNA1 (Supplementary figure 1B) The cell culture system used E8 Flex Type of the Genetic Modification Induced mutation: CTG18.1 biallelic excision (continued on next page) * Corresponding author. ..

    Transgenic Assay:

    Article Title: Biallelic excision of the CTG18.1 expansion in two Fuchs endothelial corneal dystrophy-derived iPSC lines and one control (SCTCi046-A-1, SCTCi047-A-1 and SCTCi041-A-1) using an episomal vector-based CRISPR/Cas9 approach.
    Article Snippet: .. Resource Table (continued ) Associated disease CORNEAL DYSTROPHY, FUCHS ENDOTHELIAL, 3; FECD3 OMIM #613267 Gene/locus modified in the reported transgenic line TCF4-CTG18.1 Cytogenetic location: 18q21.2 Genomic coordinates (GRCh38): (GRCh38): 18:55222185-55635957 Method of modification / usercustomisable nucleases (UCN) used, the resource used for design optimisation UCN: CRISPR/Cas, SpCas9 Software used for choosing gRNA: CRISPOR User-customisable nuclease (UCN) delivery method Encoding plasmid transfection All double-stranded DNA genetic material molecules introduced into the cells Reprogramming plasmids: pCE-hSK 11.9 kbp (Addgene #41814) pCE-hUL 11.2 kbp (Addgene #41855) pCE-hOCT3/4 10.5 kbp (Addgene #41813) CRISPR/Cas9 gRNA plasmids: pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene #62988) pSpCas9(BB)-2A-GFP (PX458) (Addgene #48138) Evidence of the absence of random integration of any plasmids or DS DNA introduced into the cells. .. Reprogramming plasmids: EBNA1-PCR CRISPR/Cas9 gRNA plasmids: CRISPR/Cas9-PCR Analysis of the nuclease-targeted allele status Sanger sequencing of the targeted alleles Homozygous allele status validation N/A Method of the off-target nuclease activity prediction and surveillance Targeted the top 4 off-target genomic site predicted by CRISPOR for both gRNA-01 and gRNA-02 Descriptive name of the transgene N/A Eukaryotic selective agent resistance cassettes (including inducible, gene/cell type-specific) N/A Inducible/constitutive expression system details N/A Date archived/stock creation date 26th August 2023 Cell line repository/bank N/A Ethical/GMO work approvals This study was GMO approved by Radboudumc (IG05-145) Addgene/public access repository recombinant DNA sources’ disclaimers (if applicable) N/A

    CRISPR:

    Article Title: Biallelic excision of the CTG18.1 expansion in two Fuchs endothelial corneal dystrophy-derived iPSC lines and one control (SCTCi046-A-1, SCTCi047-A-1 and SCTCi041-A-1) using an episomal vector-based CRISPR/Cas9 approach.
    Article Snippet: .. Resource Table (continued ) Associated disease CORNEAL DYSTROPHY, FUCHS ENDOTHELIAL, 3; FECD3 OMIM #613267 Gene/locus modified in the reported transgenic line TCF4-CTG18.1 Cytogenetic location: 18q21.2 Genomic coordinates (GRCh38): (GRCh38): 18:55222185-55635957 Method of modification / usercustomisable nucleases (UCN) used, the resource used for design optimisation UCN: CRISPR/Cas, SpCas9 Software used for choosing gRNA: CRISPOR User-customisable nuclease (UCN) delivery method Encoding plasmid transfection All double-stranded DNA genetic material molecules introduced into the cells Reprogramming plasmids: pCE-hSK 11.9 kbp (Addgene #41814) pCE-hUL 11.2 kbp (Addgene #41855) pCE-hOCT3/4 10.5 kbp (Addgene #41813) CRISPR/Cas9 gRNA plasmids: pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene #62988) pSpCas9(BB)-2A-GFP (PX458) (Addgene #48138) Evidence of the absence of random integration of any plasmids or DS DNA introduced into the cells. .. Reprogramming plasmids: EBNA1-PCR CRISPR/Cas9 gRNA plasmids: CRISPR/Cas9-PCR Analysis of the nuclease-targeted allele status Sanger sequencing of the targeted alleles Homozygous allele status validation N/A Method of the off-target nuclease activity prediction and surveillance Targeted the top 4 off-target genomic site predicted by CRISPOR for both gRNA-01 and gRNA-02 Descriptive name of the transgene N/A Eukaryotic selective agent resistance cassettes (including inducible, gene/cell type-specific) N/A Inducible/constitutive expression system details N/A Date archived/stock creation date 26th August 2023 Cell line repository/bank N/A Ethical/GMO work approvals This study was GMO approved by Radboudumc (IG05-145) Addgene/public access repository recombinant DNA sources’ disclaimers (if applicable) N/A

    Software:

    Article Title: Biallelic excision of the CTG18.1 expansion in two Fuchs endothelial corneal dystrophy-derived iPSC lines and one control (SCTCi046-A-1, SCTCi047-A-1 and SCTCi041-A-1) using an episomal vector-based CRISPR/Cas9 approach.
    Article Snippet: .. Resource Table (continued ) Associated disease CORNEAL DYSTROPHY, FUCHS ENDOTHELIAL, 3; FECD3 OMIM #613267 Gene/locus modified in the reported transgenic line TCF4-CTG18.1 Cytogenetic location: 18q21.2 Genomic coordinates (GRCh38): (GRCh38): 18:55222185-55635957 Method of modification / usercustomisable nucleases (UCN) used, the resource used for design optimisation UCN: CRISPR/Cas, SpCas9 Software used for choosing gRNA: CRISPOR User-customisable nuclease (UCN) delivery method Encoding plasmid transfection All double-stranded DNA genetic material molecules introduced into the cells Reprogramming plasmids: pCE-hSK 11.9 kbp (Addgene #41814) pCE-hUL 11.2 kbp (Addgene #41855) pCE-hOCT3/4 10.5 kbp (Addgene #41813) CRISPR/Cas9 gRNA plasmids: pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene #62988) pSpCas9(BB)-2A-GFP (PX458) (Addgene #48138) Evidence of the absence of random integration of any plasmids or DS DNA introduced into the cells. .. Reprogramming plasmids: EBNA1-PCR CRISPR/Cas9 gRNA plasmids: CRISPR/Cas9-PCR Analysis of the nuclease-targeted allele status Sanger sequencing of the targeted alleles Homozygous allele status validation N/A Method of the off-target nuclease activity prediction and surveillance Targeted the top 4 off-target genomic site predicted by CRISPOR for both gRNA-01 and gRNA-02 Descriptive name of the transgene N/A Eukaryotic selective agent resistance cassettes (including inducible, gene/cell type-specific) N/A Inducible/constitutive expression system details N/A Date archived/stock creation date 26th August 2023 Cell line repository/bank N/A Ethical/GMO work approvals This study was GMO approved by Radboudumc (IG05-145) Addgene/public access repository recombinant DNA sources’ disclaimers (if applicable) N/A

    Plasmid Preparation:

    Article Title: Biallelic excision of the CTG18.1 expansion in two Fuchs endothelial corneal dystrophy-derived iPSC lines and one control (SCTCi046-A-1, SCTCi047-A-1 and SCTCi041-A-1) using an episomal vector-based CRISPR/Cas9 approach.
    Article Snippet: .. Resource Table (continued ) Associated disease CORNEAL DYSTROPHY, FUCHS ENDOTHELIAL, 3; FECD3 OMIM #613267 Gene/locus modified in the reported transgenic line TCF4-CTG18.1 Cytogenetic location: 18q21.2 Genomic coordinates (GRCh38): (GRCh38): 18:55222185-55635957 Method of modification / usercustomisable nucleases (UCN) used, the resource used for design optimisation UCN: CRISPR/Cas, SpCas9 Software used for choosing gRNA: CRISPOR User-customisable nuclease (UCN) delivery method Encoding plasmid transfection All double-stranded DNA genetic material molecules introduced into the cells Reprogramming plasmids: pCE-hSK 11.9 kbp (Addgene #41814) pCE-hUL 11.2 kbp (Addgene #41855) pCE-hOCT3/4 10.5 kbp (Addgene #41813) CRISPR/Cas9 gRNA plasmids: pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene #62988) pSpCas9(BB)-2A-GFP (PX458) (Addgene #48138) Evidence of the absence of random integration of any plasmids or DS DNA introduced into the cells. .. Reprogramming plasmids: EBNA1-PCR CRISPR/Cas9 gRNA plasmids: CRISPR/Cas9-PCR Analysis of the nuclease-targeted allele status Sanger sequencing of the targeted alleles Homozygous allele status validation N/A Method of the off-target nuclease activity prediction and surveillance Targeted the top 4 off-target genomic site predicted by CRISPOR for both gRNA-01 and gRNA-02 Descriptive name of the transgene N/A Eukaryotic selective agent resistance cassettes (including inducible, gene/cell type-specific) N/A Inducible/constitutive expression system details N/A Date archived/stock creation date 26th August 2023 Cell line repository/bank N/A Ethical/GMO work approvals This study was GMO approved by Radboudumc (IG05-145) Addgene/public access repository recombinant DNA sources’ disclaimers (if applicable) N/A

    Transfection:

    Article Title: Biallelic excision of the CTG18.1 expansion in two Fuchs endothelial corneal dystrophy-derived iPSC lines and one control (SCTCi046-A-1, SCTCi047-A-1 and SCTCi041-A-1) using an episomal vector-based CRISPR/Cas9 approach.
    Article Snippet: .. Resource Table (continued ) Associated disease CORNEAL DYSTROPHY, FUCHS ENDOTHELIAL, 3; FECD3 OMIM #613267 Gene/locus modified in the reported transgenic line TCF4-CTG18.1 Cytogenetic location: 18q21.2 Genomic coordinates (GRCh38): (GRCh38): 18:55222185-55635957 Method of modification / usercustomisable nucleases (UCN) used, the resource used for design optimisation UCN: CRISPR/Cas, SpCas9 Software used for choosing gRNA: CRISPOR User-customisable nuclease (UCN) delivery method Encoding plasmid transfection All double-stranded DNA genetic material molecules introduced into the cells Reprogramming plasmids: pCE-hSK 11.9 kbp (Addgene #41814) pCE-hUL 11.2 kbp (Addgene #41855) pCE-hOCT3/4 10.5 kbp (Addgene #41813) CRISPR/Cas9 gRNA plasmids: pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene #62988) pSpCas9(BB)-2A-GFP (PX458) (Addgene #48138) Evidence of the absence of random integration of any plasmids or DS DNA introduced into the cells. .. Reprogramming plasmids: EBNA1-PCR CRISPR/Cas9 gRNA plasmids: CRISPR/Cas9-PCR Analysis of the nuclease-targeted allele status Sanger sequencing of the targeted alleles Homozygous allele status validation N/A Method of the off-target nuclease activity prediction and surveillance Targeted the top 4 off-target genomic site predicted by CRISPOR for both gRNA-01 and gRNA-02 Descriptive name of the transgene N/A Eukaryotic selective agent resistance cassettes (including inducible, gene/cell type-specific) N/A Inducible/constitutive expression system details N/A Date archived/stock creation date 26th August 2023 Cell line repository/bank N/A Ethical/GMO work approvals This study was GMO approved by Radboudumc (IG05-145) Addgene/public access repository recombinant DNA sources’ disclaimers (if applicable) N/A



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    pce hul 11 2 kbp  (Addgene inc)
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    Addgene inc pce hul 11 2 kbp
    Pce Hul 11 2 Kbp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pce+hul+11+2+kbp/pCE-hSK+(Plasmid+%2341814)/pm41344296-10-120-123
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    pce hul 11 2 kbp - by Bioz Stars, 2026-09
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